全脂乳清蛋白分析whey proteins from whole milk
conditions
column: ace 3 c4-300
dimensions: 150 x 2.1 mm
part number: ace-213-1502
mobile phase: a: 0.5% formic acid in h2o
b: 0.5% formic acid in mecn
gradient:
time(mins)
%b
0
35
16
43
17
80
20
80
21
35
31
35
flow rate: 0.4 ml/min
injection: 10 μl
temperature: 40 °c
detection: esi-ms (+ve)
analytes
1. α-lactalbumin
2. β-lactoglobulin b
3. β-lactoglobulin a